Multicolor fluorescent imaging by space-constrained computational spectral imaging

Computational Snapshot Spectral Imaging

Multicolor super-resolution imaging with photo-switchable fluorescent probes.

Recent advances in far-field optical nanoscopy have enabled fluorescence imaging with a spatial resolution of 20 to 50 nanometers. Multicolor super-resolution imaging, however, remains a challenging task. Here, we introduce a family of photo-switchable fluorescent probes and demonstrate multicolor stochastic optical reconstruction microscopy (STORM). Each probe consists of a photo-switchable "r...

Multicolor Immunofluorescence Imaging

Protocols for immunolabeling vary widely due to experimental goals. Interactions that contribute to antibody binding include hydrogen bonds, ionic bonds, hydrophobic interactions and van der Waals forces. The strength of each of these interactions is weak compared to a covalent bond. Consequently, a large number of noncovalent interactions are required to stabilize antibody binding to an antige...

Spectral Imaging: a Powerful Tool for Confocal Multicolor Imaging in Living Plant Cells

Multiscale reconstruction for computational spectral imaging

In this work we develop a spectral imaging system and associated reconstruction methods that have been designed to exploit the theory of compressive sensing. Recent work in this emerging field indicates that when the signal of interest is very sparse (i.e. zero-valued at most locations) or highly compressible in some basis, relatively few incoherent observations are necessary to reconstruct the...